Cloning of InvG gene from Salmonella Enterica in the Expression Vector pET32a and Evaluation of its Expression in hosts BL21- DE3
Clicks: 158
ID: 109682
2015
Salmonella is one of the causes of infectious diseases as a common disease in human and animals, has important health and economic terms, which has so far of a vaccine for it not been established. The InvG gene due to structural and Type three secretion systems as one of the primary genes located in the membrane of Bacteria also play a major role in the initial binding of the Bacteria to the host cell. The main aim of the present study was to introduce recombinant protein expression InvG as an effective adjuvant and a DNA vaccine. The design makes use of specific primers and reaction PCR, InvG gene was amplified Salmonella. After purification, the gene in the plasmid vector pET32a(+) cloned for expression. The recombinant plasmid pET32a-InvG into Escherichia coli strain became BL21-DE3. To produce recombinant protein expression system, the Bacterial expression vector containing the target gene (InvG) induced using IPTG. The results of sequencing showed that the sequence of the amplified gene cloned into pET32a expression vector for gene InvG with sequences recorded in the same gene bank. Recombinant protein production with IPTG induction to vector containing the plasmid pET32a-InvG performed successfully. Confirmation of InvG gene expression in the expression system performed by SDS-PAGE and Dot blotting analysis. The present study showed that the production of InvG recombinant in E. coli hosts is possible and protein with a weight of about 81 kDa was produced.
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jahandar2015cloning
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| Authors | Jahandar, Mohammad Hassan;Nasiri, Mohmmad Reza;Nezhad, Ali Asghar Aslami;Pour, Mojtaba Tahmoures;parast, Alireza Haq; |
| Journal | مجله بیوتکنولوژی کشاورزی |
| Year | 2015 |
| DOI | DOI not found |
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