Reconstitution of cytomegalovirus-specific T-cell immunity following unmanipulated haploidentical allogeneic hematopoietic stem cell transplantation with posttransplant cyclophosphamide.

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2020
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Abstract
Cytomegalovirus (CMV) DNAemia and CMV disease have been reported as more frequent in patients undergoing haploidentical allogeneic hematopoietic stem cell transplantation (Haplo-HSCT) than in those receiving HLA-matched allografts. This could be due to impaired CMV-specific T-cell reconstitution. Here, we conducted a multicenter observational study to assess CMV pp65 and IE-1-specific T cells kinetics in patients undergoing unmanipulated Haplo-HSCT with posttransplant cyclophosphamide (PT/Cy-haplo) and compared it with patients allografted with HLA-matched donors. Plasma CMV DNA load was monitored by real-time PCR and enumeration of CMV-specific IFN-γ-producing CD8 and CD4 T cells was performed by flow cytometry for intracellular cytokine staining at days +30, +60, +90, and +180 after transplantation. CMV DNAemia developed in 62 patients, occurring with comparable frequency in PT/Cy-haplo and MRD/MUD recipients (P = 0.14). There were no significant differences across groups in the number of patients either displaying detectable CMV-specific CD8 and CD4 T-cell responses or acquiring CMV-specific T-cell levels conferring protection against subsequent infection. CMV-specific T-cell counts were comparable between groups at most time points examined, irrespective of whether CMV DNAemia occurred or not prior to monitoring. Collectively the data suggest that PT/Cy-haplo recipients may reconstitute CMV-specific T-cell immunity to the same extent as patients undergoing HLA-matched allo-HSCT.
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huntley2020reconstitutionbone Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Huntley, Dixie;Giménez, Estela;Pascual, María Jesús;Remigia, María José;Amat, Paula;Vázquez, Lourdes;Hernández, Marta;Hernández-Boluda, Juan Carlos;Gago, Beatriz;Piñana, José Luis;García, Magdalena;Martínez, Ariadna;Mateo, Eva;Gozalbo-Rovira, Roberto;Albert, Eliseo;Solano, Carlos;Navarro, David;
Journal bone marrow transplantation
Year 2020
DOI 10.1038/s41409-020-0865-x
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Keywords
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